KMID : 0861020080230010085
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Korea Journal of Herbology 2008 Volume.23 No. 1 p.85 ~ p.92
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Preparation of Black Panax Ginseng by New Methods and its Antitumor Activity
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Kim Eui-Keom
Lee Jee-Hyun Shen Gui-nan Cho Soo-Hyun Myung Chang-Seon Song Gyu-Yong Park Yong-Jin Seo Young-Bae Kim Dong-Hee Roh Seong-Soo Kim Long-Guo Oh Han-Jin Yun Jae-Don
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Abstract
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Objectives: This study was performed to efficiently make Black Panax Ginseng (BPG) and evaluate its
antitumor activity.
Methods: Panax ginseng was steamed at 95¡É for 3 h, dried and steamed again at 115¡É for 6 h. The
main ginsenosides of BPG were Rg3, Rk1 and Rg5.
Results: Among the saponins in BPG, the amount of ginsenoside Rg3 was determined by HPLC method.
The 11.48 §· of ginsenoside Rg3 was obtained from 1g of dried BPG. The crude saponin fraction (CSF)
of BPG was tested in vitro for its cytotoxic activities against various human cancer cell lines, such as
ACHN, NCI-H23, HCT-15 and PC-3. The CSF of BPG exhibited stronger cytotoxic activity than that of
red Panax ginsneng. CSF of BPG exhibited good cytotoxic activities against ACHN, HCT-15, and PC-3
cell lines with IC50 values of 60.3-90.8 §¶/§¢. However, CSF of BPG did not show any cytotoxic activity
against NCI-H23 cell line.
Conclusions: BPG produced by new manufacturing is more effective than BPG produced by existing
processing in anticancer activity. And new BPG has a possibility of investigation because of high
contents of Rg3, Rk1 and Rg5 that have various phisological activities.
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KEYWORD
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Black Panax Ginseng, Antitumor effect, Ginsenoside Rg3
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